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Preparation for para-uterine imaging (A) Preparatory Steps 1 - 3: Setup prior to the start of surgery. 1: Mouse temperature controller, with associated heating pad and temperature probe. 2: Shaver. 3: UV-protective glasses. 4: Norland 65 UV-curing glue. 5: Eye lubricant. 6: Cover glasses. 7: Plastic pipette. 8: Retractors (x4). 9: Embryo holder. 10: Headbar. 11: Fine scissors. 12: Spring scissors. <t>13:</t> <t>Blunt-tipped</t> forceps (x2). 14: Ring-tipped forceps. 15: Fine-tipped forceps (x2). 16: Binocular dissection scope. 17: HBSS. 18: Alligator clamp. 19: Underpad. More details in A. (B – L) Stepwise progression of surgery. (B) Preparatory Steps 8, 9 and Steps 1a, 1b: Placement of the dam in a supine position, followed by sterilization, removal of fur from the abdomen, and cutting through the skin. (C) Steps 1a, 1b: Cutting through the muscular fascia. (D) Step 1c, 3: Using retractors to open the abdominal cavity, exposing the uterine horns, and opening up the uterus. (E) Steps 4: Selecting an embryo and then opening up the amniotic sac to expose the embryo. Preparing an alligator clamp to stabilize the embryo holder. (F) Step 7: Placing the embryo gently in the embryo holder. (G) Step 9: Transferring the embryo holder to the alligator clamp. (H) Step 11: Adding agar to the embryo holder. (I) Step 12: Adhering the headbar to the embryo holder. (J) Step 13: Transferring the embryo holder to the external supports. (K) Steps 15, 16: Removing the skin (and bone) from the head of the embryo. Inset: Exposed embryonic brain (process detailed in ). (L) Step 19: Cover glass added to the embryo. Inset: Embryonic brain covered by cover glass, with the space filled with cortex buffer (process detailed in ).
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Preparation for para-uterine imaging (A) Preparatory Steps 1 - 3: Setup prior to the start of surgery. 1: Mouse temperature controller, with associated heating pad and temperature probe. 2: Shaver. 3: UV-protective glasses. 4: Norland 65 UV-curing glue. 5: Eye lubricant. 6: Cover glasses. 7: Plastic pipette. 8: Retractors (x4). 9: Embryo holder. 10: Headbar. 11: Fine scissors. 12: Spring scissors. <t>13:</t> <t>Blunt-tipped</t> forceps (x2). 14: Ring-tipped forceps. 15: Fine-tipped forceps (x2). 16: Binocular dissection scope. 17: HBSS. 18: Alligator clamp. 19: Underpad. More details in A. (B – L) Stepwise progression of surgery. (B) Preparatory Steps 8, 9 and Steps 1a, 1b: Placement of the dam in a supine position, followed by sterilization, removal of fur from the abdomen, and cutting through the skin. (C) Steps 1a, 1b: Cutting through the muscular fascia. (D) Step 1c, 3: Using retractors to open the abdominal cavity, exposing the uterine horns, and opening up the uterus. (E) Steps 4: Selecting an embryo and then opening up the amniotic sac to expose the embryo. Preparing an alligator clamp to stabilize the embryo holder. (F) Step 7: Placing the embryo gently in the embryo holder. (G) Step 9: Transferring the embryo holder to the alligator clamp. (H) Step 11: Adding agar to the embryo holder. (I) Step 12: Adhering the headbar to the embryo holder. (J) Step 13: Transferring the embryo holder to the external supports. (K) Steps 15, 16: Removing the skin (and bone) from the head of the embryo. Inset: Exposed embryonic brain (process detailed in ). (L) Step 19: Cover glass added to the embryo. Inset: Embryonic brain covered by cover glass, with the space filled with cortex buffer (process detailed in ).
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Preparation for para-uterine imaging (A) Preparatory Steps 1 - 3: Setup prior to the start of surgery. 1: Mouse temperature controller, with associated heating pad and temperature probe. 2: Shaver. 3: UV-protective glasses. 4: Norland 65 UV-curing glue. 5: Eye lubricant. 6: Cover glasses. 7: Plastic pipette. 8: Retractors (x4). 9: Embryo holder. 10: Headbar. 11: Fine scissors. 12: Spring scissors. <t>13:</t> <t>Blunt-tipped</t> forceps (x2). 14: Ring-tipped forceps. 15: Fine-tipped forceps (x2). 16: Binocular dissection scope. 17: HBSS. 18: Alligator clamp. 19: Underpad. More details in A. (B – L) Stepwise progression of surgery. (B) Preparatory Steps 8, 9 and Steps 1a, 1b: Placement of the dam in a supine position, followed by sterilization, removal of fur from the abdomen, and cutting through the skin. (C) Steps 1a, 1b: Cutting through the muscular fascia. (D) Step 1c, 3: Using retractors to open the abdominal cavity, exposing the uterine horns, and opening up the uterus. (E) Steps 4: Selecting an embryo and then opening up the amniotic sac to expose the embryo. Preparing an alligator clamp to stabilize the embryo holder. (F) Step 7: Placing the embryo gently in the embryo holder. (G) Step 9: Transferring the embryo holder to the alligator clamp. (H) Step 11: Adding agar to the embryo holder. (I) Step 12: Adhering the headbar to the embryo holder. (J) Step 13: Transferring the embryo holder to the external supports. (K) Steps 15, 16: Removing the skin (and bone) from the head of the embryo. Inset: Exposed embryonic brain (process detailed in ). (L) Step 19: Cover glass added to the embryo. Inset: Embryonic brain covered by cover glass, with the space filled with cortex buffer (process detailed in ).
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Preparation for para-uterine imaging (A) Preparatory Steps 1 - 3: Setup prior to the start of surgery. 1: Mouse temperature controller, with associated heating pad and temperature probe. 2: Shaver. 3: UV-protective glasses. 4: Norland 65 UV-curing glue. 5: Eye lubricant. 6: Cover glasses. 7: Plastic pipette. 8: Retractors (x4). 9: Embryo holder. 10: Headbar. 11: Fine scissors. 12: Spring scissors. <t>13:</t> <t>Blunt-tipped</t> forceps (x2). 14: Ring-tipped forceps. 15: Fine-tipped forceps (x2). 16: Binocular dissection scope. 17: HBSS. 18: Alligator clamp. 19: Underpad. More details in A. (B – L) Stepwise progression of surgery. (B) Preparatory Steps 8, 9 and Steps 1a, 1b: Placement of the dam in a supine position, followed by sterilization, removal of fur from the abdomen, and cutting through the skin. (C) Steps 1a, 1b: Cutting through the muscular fascia. (D) Step 1c, 3: Using retractors to open the abdominal cavity, exposing the uterine horns, and opening up the uterus. (E) Steps 4: Selecting an embryo and then opening up the amniotic sac to expose the embryo. Preparing an alligator clamp to stabilize the embryo holder. (F) Step 7: Placing the embryo gently in the embryo holder. (G) Step 9: Transferring the embryo holder to the alligator clamp. (H) Step 11: Adding agar to the embryo holder. (I) Step 12: Adhering the headbar to the embryo holder. (J) Step 13: Transferring the embryo holder to the external supports. (K) Steps 15, 16: Removing the skin (and bone) from the head of the embryo. Inset: Exposed embryonic brain (process detailed in ). (L) Step 19: Cover glass added to the embryo. Inset: Embryonic brain covered by cover glass, with the space filled with cortex buffer (process detailed in ).
Fisherbrand Pipette Specific Tips 1000, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fisherbrand pipette specific tips 1000/product/Fisher Scientific
Average 86 stars, based on 1 article reviews
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blunt tips  (Kent Scientific Corp)
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Preparation for para-uterine imaging (A) Preparatory Steps 1 - 3: Setup prior to the start of surgery. 1: Mouse temperature controller, with associated heating pad and temperature probe. 2: Shaver. 3: UV-protective glasses. 4: Norland 65 UV-curing glue. 5: Eye lubricant. 6: Cover glasses. 7: Plastic pipette. 8: Retractors (x4). 9: Embryo holder. 10: Headbar. 11: Fine scissors. 12: Spring scissors. <t>13:</t> <t>Blunt-tipped</t> forceps (x2). 14: Ring-tipped forceps. 15: Fine-tipped forceps (x2). 16: Binocular dissection scope. 17: HBSS. 18: Alligator clamp. 19: Underpad. More details in A. (B – L) Stepwise progression of surgery. (B) Preparatory Steps 8, 9 and Steps 1a, 1b: Placement of the dam in a supine position, followed by sterilization, removal of fur from the abdomen, and cutting through the skin. (C) Steps 1a, 1b: Cutting through the muscular fascia. (D) Step 1c, 3: Using retractors to open the abdominal cavity, exposing the uterine horns, and opening up the uterus. (E) Steps 4: Selecting an embryo and then opening up the amniotic sac to expose the embryo. Preparing an alligator clamp to stabilize the embryo holder. (F) Step 7: Placing the embryo gently in the embryo holder. (G) Step 9: Transferring the embryo holder to the alligator clamp. (H) Step 11: Adding agar to the embryo holder. (I) Step 12: Adhering the headbar to the embryo holder. (J) Step 13: Transferring the embryo holder to the external supports. (K) Steps 15, 16: Removing the skin (and bone) from the head of the embryo. Inset: Exposed embryonic brain (process detailed in ). (L) Step 19: Cover glass added to the embryo. Inset: Embryonic brain covered by cover glass, with the space filled with cortex buffer (process detailed in ).
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Image Search Results


Preparation for para-uterine imaging (A) Preparatory Steps 1 - 3: Setup prior to the start of surgery. 1: Mouse temperature controller, with associated heating pad and temperature probe. 2: Shaver. 3: UV-protective glasses. 4: Norland 65 UV-curing glue. 5: Eye lubricant. 6: Cover glasses. 7: Plastic pipette. 8: Retractors (x4). 9: Embryo holder. 10: Headbar. 11: Fine scissors. 12: Spring scissors. 13: Blunt-tipped forceps (x2). 14: Ring-tipped forceps. 15: Fine-tipped forceps (x2). 16: Binocular dissection scope. 17: HBSS. 18: Alligator clamp. 19: Underpad. More details in A. (B – L) Stepwise progression of surgery. (B) Preparatory Steps 8, 9 and Steps 1a, 1b: Placement of the dam in a supine position, followed by sterilization, removal of fur from the abdomen, and cutting through the skin. (C) Steps 1a, 1b: Cutting through the muscular fascia. (D) Step 1c, 3: Using retractors to open the abdominal cavity, exposing the uterine horns, and opening up the uterus. (E) Steps 4: Selecting an embryo and then opening up the amniotic sac to expose the embryo. Preparing an alligator clamp to stabilize the embryo holder. (F) Step 7: Placing the embryo gently in the embryo holder. (G) Step 9: Transferring the embryo holder to the alligator clamp. (H) Step 11: Adding agar to the embryo holder. (I) Step 12: Adhering the headbar to the embryo holder. (J) Step 13: Transferring the embryo holder to the external supports. (K) Steps 15, 16: Removing the skin (and bone) from the head of the embryo. Inset: Exposed embryonic brain (process detailed in ). (L) Step 19: Cover glass added to the embryo. Inset: Embryonic brain covered by cover glass, with the space filled with cortex buffer (process detailed in ).

Journal: STAR Protocols

Article Title: Para-uterine imaging protocol: Stabilizing living mouse embryos within the maternal abdomen for in vivo imaging and visually guided physiology

doi: 10.1016/j.xpro.2026.104446

Figure Lengend Snippet: Preparation for para-uterine imaging (A) Preparatory Steps 1 - 3: Setup prior to the start of surgery. 1: Mouse temperature controller, with associated heating pad and temperature probe. 2: Shaver. 3: UV-protective glasses. 4: Norland 65 UV-curing glue. 5: Eye lubricant. 6: Cover glasses. 7: Plastic pipette. 8: Retractors (x4). 9: Embryo holder. 10: Headbar. 11: Fine scissors. 12: Spring scissors. 13: Blunt-tipped forceps (x2). 14: Ring-tipped forceps. 15: Fine-tipped forceps (x2). 16: Binocular dissection scope. 17: HBSS. 18: Alligator clamp. 19: Underpad. More details in A. (B – L) Stepwise progression of surgery. (B) Preparatory Steps 8, 9 and Steps 1a, 1b: Placement of the dam in a supine position, followed by sterilization, removal of fur from the abdomen, and cutting through the skin. (C) Steps 1a, 1b: Cutting through the muscular fascia. (D) Step 1c, 3: Using retractors to open the abdominal cavity, exposing the uterine horns, and opening up the uterus. (E) Steps 4: Selecting an embryo and then opening up the amniotic sac to expose the embryo. Preparing an alligator clamp to stabilize the embryo holder. (F) Step 7: Placing the embryo gently in the embryo holder. (G) Step 9: Transferring the embryo holder to the alligator clamp. (H) Step 11: Adding agar to the embryo holder. (I) Step 12: Adhering the headbar to the embryo holder. (J) Step 13: Transferring the embryo holder to the external supports. (K) Steps 15, 16: Removing the skin (and bone) from the head of the embryo. Inset: Exposed embryonic brain (process detailed in ). (L) Step 19: Cover glass added to the embryo. Inset: Embryonic brain covered by cover glass, with the space filled with cortex buffer (process detailed in ).

Article Snippet: Blunt-tipped forceps , Fine Science Tools , 11652–10.

Techniques: Imaging, Transferring, Dissection